Laboratory technician for the research group “RNA-based Regulation of Gene Expression”

A position for a laboratory technician/manager is available in the research group of Peter J Lukavsky, PhD “RNA-based Regulation of Gene Expression” which is part of Structural Biology program at CEITEC, Masaryk University (MU). We study disease-related biological processes by investigating protein structures and their interactions with other proteins, RNA or small molecule inhibitors using biomolecular NMR and related biophysical methods.

Job description:
• Expression and purification of proteins
• Transcription and purification of RNAs
• Preparation of various media and buffers
• PCR and cloning of RNA and protein constructs
• Preparation of isotope-labelled nucleotides
• Binding studies using ITC, fluorescence anisotropy, EMSA, and other biophysical techniques including data analysis
• Performance of NMR experiments to study small molecule interactions with proteins and RNA
• Implementing new techniques and projects
• Help incoming co-workers into a smooth start in the lab
• Ordering of chemicals and lab supplies

Requirements:
• At least Master degree in molecular biology, biochemistry, biophysics, chemistry or other related fields of life-sciences
• Good knowledge of English (oral and written)
• Excellent interpersonal and communication skills
• High motivation, flexibility and self-driven interest to learn new things
• Reliability, team player, meticulousness
• Knowledge of "laboratory chemistry" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures etc.
• Computer literacy, MS word, Excel, Adobe Illustrator.
• Excellent experience with molecular biology, protein and RNA purification, biochemistry and NMR spectroscopy is a big plus

We offer:
• Interesting work in a top scientific team
• Stable base and excellent instrumental equipment of CEITEC-MU
• MU employment benefits
• Salary based on qualification and experience; part time appointment possible


Please send CV and a short motivational letter including: a) description of previous experiences b) technical skills and c) at least two contact references should be sent by e-application available on https://www.muni.cz/en/about-us/careers/vacancies/66999.

In the case of any difficulties filling out the e-application, please contact Veronika Slezáková ****
Deadline of the applications is 31 August 2022.